Table 1 Primary sequences of EF-hand Ca2+-binding loops from EfhP and CaM.

From: EF-hand protein, EfhP, specifically binds Ca2+ and mediates Ca2+ regulation of virulence in a human pathogen Pseudomonas aeruginosa

Protein

Ca2+-binding loop

+X

1

2

+Y

3

4

+Z

5

6

−Y

7

8

−X

9

10

11

−Z

12

EfhP

I

D

T

D

H

D

G

K

V

S

R

A

E99

 

II

D

S

D

H

D

G

F

I

S

E

A

E126

CaM

I

D

K

D

G

D

G

T

I

T

T

K

E31

 

II

D

A

D

G

N

G

T

I

D

F

P

E67

 

III

D

K

D

G

N

G

Y

I

S

A

A

E104

 

IV

D

L

D

G

D

G

G

V

N

Y

E

E140

  1. Ca2+-binding sites are numbered, Ca2+-coordinating residues are labeled as sites X, Y, Z, −Y, −X, and −Z. Identical residues in EfhP loops are shown in bold, identical residues in EfhP and CaM are in italics. The 12th glutamate residues are shown with the coordinates in the corresponding proteins.
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