Table 1 River and swamp buffalo secondary oocytes matured in vitro and analysed by sequential M-FISH with 6 chromosome specific painting probes for aneuploidy detection.

From: Oocyte aneuploidy rates in river and swamp buffalo types (Bubalus bubalis) determined by Multi-color Fluorescence In Situ Hybridization (M-FISH)

Type

Breed

Number of analysed oocytes

Selected for IVM

Total MII analysed (a)

Unreduced (% on a)

Reduced

Aneuploid

PSSC (% on c)

Total (b) (% on a)

− PB (% on b)

+ PB (c) (% on b)

Nullisomic (% on c)

Disomic (% on c)

Total (% on c)

River

Murrah

60

51

1 (1.96)

50 (98.03)

5 (10.00)

45 (90.00)

–

1x

1

–

Swamp

Local

60

53

1 (1.88)

52 (98.11)

8 (15.38)

44 (84.61)

1x + 15

–

2

1x

 

Total

120

104

2 (1.92)

102 (98.07)

13 (12.74)

89 (87.25)

2 (2.25)

1 (1.12)

3 (3.37)

1 (1.12)

  1. IVM in vitro maturation, PB polar body, PSSC premature separation of sister chromatids.
  2. (a) Number of matured oocytes;
  3. (b) Number of oocytes with reduced chromosome number;
  4. (c) Number of oocytes with reduced chromosome number and analysable PB.
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