Table 1 Verified conditions for SEM observation.

From: The new preparation method for paraffin-embedded samples applying scanning electron microscopy revealed characteristic features in asthma-induced mice

1. Glass slides

PM (Matsunami)

EM (Nissin EM)

2. Os coating thichness

2.5 nm

5.0 nm

3. SEM acceleration voltage

5kv and 10kv

10kv and 15kv

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