Figure 1

IA vs. double immunofluorescence of macrophage identification. Panel (A) and (B) show PD-L1 stained tumor field of view with and with out the macrophage algorithm labeling (red = PD-L1 positive; blue = PD-L1 negative). Panel (C) and (D) represent corresponding field of view in a serial section stained with CD68 (green) and CD163 (red).