Figure 6 | Scientific Reports

Figure 6

From: Polyploid giant cancer cells are dependent on cholesterol for progeny formation through amitotic division

Figure 6

Ceramide and cholesterol segregate, with ceramide appearing at periphery of PGCC treated with LCL521 or simvastatin. (A) Uncropped 63X images were obtained of parental cells or PGCC treated with 5 µM LCL521 or 1 µM simvastatin for 48 h. Green indicates ceramide staining, which appears at cellular termini in treated PGCC. Blue indicates filipin staining of cholesterol and red indicates SYTOX deep red staining of nucleic acids. (B) Quantification of ceramide/cholesterol signal colocalization across all samples and conditions, showing a durable lack of colocalization for the lipids.

Back to article page