Figure 5
Acute administration of TMAO does not impair NO production. In HAEC incubated with TMAO (10−6 M) for one hour, Western Blot protein expression revealed no differences in eNOS phosphorylation (A) at the activatory site, serine 1177, and (B) the inhibitory site, threonine 495. Similar was also the (C) endothelial NO production. (D) Endothelial tissue factor expression was not induced by TMAO incubation. (E) Summarizes the observed in-vitro effects of TMAO incubation in HAECs. Original blots/gels are presented in Supplementary Fig. 5. Unpaired T-test or 1-way ANOVA with Dunnett correction, n = 4independent cell-culture experiments with 1–2 replicates per experiment per condition. TMAO: trimethylamine N-oxide, HAECs: human aortic endothelial cells, eNOS: endothelial nitric oxide synthase, NO: nitric oxide.
