Table 3 Sensitivity of probes and its combinations for multiplex assay.

From: Boosting SARS-CoV-2 detection combining pooling and multiplex strategies

Targets (probe dye)

Sensitivity (%)

N (FAM)

99.78

E (FAM)

99.1

E (Cy5)

99.94

Orf1ab (FAM)

99.78

N (FAM)/E (Cy5)

99.78/99.94

N (FAM)/Orf1ab (Cy5)

99.97/99.98

E (FAM)/Orf1ab (Cy5)

99.58/99.97