Figure 4

Proteomic analysis shows three distinct cell populations. (A) PCA plot showing three distinct and reproducible cell populations upon global proteomic analysis of cultured ECs, PCs, and VSMCs, n = 3. (B) Boxplots (median, interquartile range), of proteomic analysis of cell-specific markers. Values were filtered based on FDR rate < 0.05 and then log fold change values compared to the other cell types, n = 3, *p < 0.05, compared to both other cell types (R software package edgeR).