Table 1 Ct values of samples as measured by qPCR

From: Longer amplicons provide better sensitivity for electrochemical sensing of viral nucleic acid in water samples using PCB electrodes

 

Ct value for \(117\,\hbox {bp}\)

Ct value for \(503\,\hbox {bp}\)

No template control (NTC)\(^{1}\)

\(31 \pm 0.04\)

\(30.75 \pm 0.44\)

Positive control (PC)\(^{2}\)

\(17.87 \pm 0.17\)

\(16.73 \pm 0.12\)

Test sample-1

\(20.65 \pm 0.11\)

\(19.41 \pm 0.08\)

Test sample-2

\(20.37 \pm 0.07\)

\(19.35 \pm 0.07\)

Test sample-3

\(21.1 \pm 0.28\)

\(20.51 \pm 0.2\)

  1. \(^1\) NTC has no template added in the reaction.
  2. \(^2\) Positive control has cDNA isolated from purified Phi6 bacteriophage particles as template.
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