Figure 1

Generation of iPSCs from fibroblasts of three breeds of miniature pigs. (A) Schematic diagram illustrating the timeline and stepwise procedures to establish iPSC lines. (B) Phase-contrast images showing flat, elongated cells with typical fibroblast morphology. (C) Phase-contrast images showing pig iPSC colonies with well-defined borders at day 21 post transfection. (D) ALP staining of iPSC colonies and corresponding reprogramming efficiency presented by the number of ALP-positive colonies per million cells. ALP-positive colonies in each 100-mm dish were counted. Scale bar = 200 µm. *p < 0.05; n = 3.