Figure 6
From: Epigenetic regulation of BAF60A determines efficiency of miniature swine iPSC generation
Expression levels of SWI/SNF complexes and STAT3 activity in cells of 3 miniature pig breeds. (A) Transcript levels of selected epigenetic enzymes in fibroblasts and iPSCs of different breeds of miniature pigs. (B) Images of western blots and quantification of protein bands detecting BAF60A, pSTAT3, and STAT3 expression in cells. GAPDH was used as a loading control. (C) Images and quantification of western blots of proteins extracted from YMS iPSCs treated with the STAT3 inhibitor CPT for 2 h. GAPDH was used as a loading control. (D) Images and quantification of western blots of proteins extracted from YMS iPSCs treated with BAF60A siRNA or scrambled control. GAPDH was used as a loading control. (E) Interaction of BAF60A and OCT4 in YMS iPSC lines analyzed by co-IP. Original western blots are presented in Supplementary Fig. S5. *p < 0.05; n = 3.
