Figure 7

Effects of BAF60A knockdown and overexpression on the reprogramming efficiency of YMS fibroblasts. (A) Transcript levels of BAF60A in scrambled control and BAF60A-knockdown iPSCs (left). Macrographs of iPSC colonies formed in Petri dishes detected by ALP staining 21 days after transfection for BAF60A knockdown (middle). Quantification of ALP-positive colonies normalized to total transfected cells (right). (B) Colony morphology of control and BAF60A-overexpressing iPSCs (top). Transcript levels of BAF60A in empty vector control and BAF60A-overexpressing iPSCs (bottom left). Macrographs of iPSC colonies detected by ALP staining 21 days after transfection for BAF60A overexpression (bottom middle). Quantification of ALP-positive colonies normalized to total transfected cells (bottom right). Scale bar = 200 µm. *p < 0.05; n = 3.