Figure 4 | Scientific Reports

Figure 4

From: Biogenic silver/silver chloride nanoparticles inhibit human cancer cells proliferation in vitro and Ehrlich ascites carcinoma cells growth in vivo

Figure 4

Cell morphological studies by the fluorometric assays. (A) Detection of apoptosis in GSCs by immunofluorometric assay. Where a1 and b1 represent the optical microscopic images of the untreated and G. densiflorum-Ag/AgCl-NPs treated GSCs, respectively; a2 and b2 represent the fluorescence microscopic images of the untreated and G. densiflorum-Ag/AgCl-NPs treated GSCs, respectively after staining with Cy3 goat-anti Rabbit IgG antibody. (B) Detection of early and late apoptosis in BxPC-3 by FITC-annexin V/PI. (C) Detection of early and late apoptosis in MCF-7 cells by FITC-annexin V/PI. In the Figures (B) and (C), a1, b1 and c1 indicate the optical microscopic images of untreated, G. densiflorum-Ag/AgCl-NPs and K. rotunda-Ag/AgCl-NPs treated cells, respectively; a2, b2 and c2 indicate the fluorescence microscopic images of untreated, G. densiflorum-Ag/AgCl-NPs and K. rotunda-Ag/AgCl-NPs treated cells, respectively after stained with FITC-annexin V; a3, b3, and c3 indicate the fluorescence microscopic images of untreated, G. densiflorum-Ag/AgCl-NPs and K. rotunda-Ag/AgCl-NPs, treated cells, respectively after stained with PI. (D) ROS generation in MCF-7 cells. Where a1, b1 and c1 represent the optical microscopic images of the untreated control, G. densiflorum-Ag/AgCl-NPs and K. rotunda-Ag/AgCl-NPs treated MCF-7 cells, respectively; a2, b2 and c2 represent the optical microscopic images of the untreated control, G. densiflorum-Ag/AgCl-NPs and K. rotunda-Ag/AgCl-NPs treated MCF-7 cells, respectively after stained with DCFH-DA. All image was captured at 20 × magnification. In Fig (A), the solid bar indicates 20 m.

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