Figure 2

Eight weeks of aerobic physical exercise in diabetic rats was associated with renal and muscle AMPK activation and an elevation in the expression of FNDC5-irisn and PGC1-α in the muscle. (A–C) Western blot analysis of pAMPK^(Thr172), AMPKα, pACC^(Ser79), ACC, and vinculin in the renal cortex followed by quantitation of pAMPK^(Thr172)/vinculin by AMPKα/vinculin ratio (A) and pACC^(Ser79)/vinculin by ACC/vinculin ratio (B). (D–H) Western blot analysis of FNDC5-irisn, GADPH, PGC-1α, vinculin, pAMPK^(Thr172), AMPKα, pACC^(Ser79), and ACC in the muscle (H), followed by quantitation of FNDC5-irisn/GADPH (D), PGC-1α/vinculin (E), pAMPK^(Thr172)/vinculin by AMPKα/vinculin (F), and pACC^(Ser79)/vinculin by ACC/vinculin ratio (G). (I) Muscle AMP/ATP ratio. (J–L) Correlations of muscle irisin vs. UACR (J) as well as muscle irisin vs. glomerular fibronectin (K) and renal acetyl NF-κB (L). Protein expression levels were normalized to vinculin or GAPDH. Blots are representative of three independent experiments. Results are means ± SE. CT, nondiabetic; DM, sedentary diabetic; DM + Exe, exercised diabetic. UACR, urinary albumin-to-creatine ratio. ACC, acetyl-CoA carboxylase. ^#p < 0.05 vs. CT; *p < 0.05 vs. DM.