Figure 1

Construction of the CHIKV PsV. (a) The CHIKV PsV construction scheme. Each gene fragment was indicated by the colored box. (b) Construction and identification of the pMD2.G-CHIKV-env. Primers for PCR were described in the “Materials and methods” section. 5 ul of each product was analyzed in 0.8% agarose gel. Lane 1, DNA marker; Lane 2, linearized pMD2.G-ΔVSV-G backbone (4295 bp); Lane 3, CHIKV envelope gene (2995 bp); Lane 4, undigested pMD2.G-CHIKV-env plasmid (7266 bp); Lane 5, pMD2.G-CHIKV-env plasmid digested by AscI and Xbal (2967 bp and 4299 bp). (c) Map of the recombinant plasmid pMD2.G-CHIKV-env that was illustrated by the software SnapGene Viewer (CA, USA).