Figure 3

Titration of the CHIKV PsV. 293T cells in the 24-well plate were transduced with serial ten-fold dilutions of PsV particles (a) or VSV-G PsV (b). At 72 h post transduction, cells were firstly observed under the fluorescence microscope (left panel). Then cells were trypsinized and resuspended for titration via flow cytometric analysis (right panel). Panels in this figure from top to bottom correspond to 1–1000 times dilutions. The MOI was calculated for 293T cells in each well according to the PsV titer. Scale bar: 100 μm.