Figure 4
From: Preparation and application of chikungunya pseudovirus containing double reporter genes
Development of CHIKV PsV micro-neutralization assay. Serially diluted serum from CHIKV-immunized rabbit was incubated with CHIKV or VSV-G PsV. After one-hour incubation, the mixture was transferred to 293T cells. Luciferase activity was measured at 72 h post transduction to evaluate the neutralizing ability of antibody to CHIKV PsV or VSV-G PsV on the microplate reader, followed by the calculation of ID50. Error bars show the standard deviations (SD) of results from duplicate independent experiments.
