Table 1 Catalytic efficiency (kcat/KM) of 4MDM with Ala-pNA, Arg-pNA, and Pro-pNA.

From: Substrate-dependent modulation of the leukotriene A4 hydrolase aminopeptidase activity and effect in a murine model of acute lung inflammation

Arg-pNA

Ala-pNA

Pro-pNA

4MDM [µM]

kcat (s−1)

KM (mM)

kcat/KM (mM−1 s−1)

4MDM [µM]

kcat (s−1)

KM (mM)

kcat/KM (mM−1 s−1)

4MDM [µM]

kcat (s−1)

KM (mM)

kcat/KM (mM−1 s−1)

0

24.35 ± 2.67

18.79 ± 2.73

1.30

0

1.74 ± 0.07

1.60 ± 0.12

1.08

0

0.19 ± 0.01

0.84 ± 0.05

0.23

10.0

24.62 ± 3.99

24.26 ± 4.95

1.02

0.1

3.01 ± 0.13

0.98 ± 0.12

3.07

100.0

0.65 ± 0.03

1.89 ± 0.25

0.34

20.0

15.59 ± 3.01

17.70 ± 4.58

0.88

0.5

5.54 ± 0.23

0.91 ± 0.11

6.06

200.0

0.93 ± 0.08

2.90 ± 0.62

0.32

40.0

24.42 ± 7.31

37.89 ± 13.19

0.64

1.0

8.68 ± 0.36

1.03 ± 0.12

8.46

400.0

1.25 ± 0.06

3.92 ± 0.40

0.32

60.0

16.22 ± 3.99

28.31 ± 8.50

0.57

5.0

23.91 ± 1.04

1.52 ± 0.16

15.71

600.0

1.36 ± 0.09

4.32 ± 0.63

0.32

80.0

12.45 ± 3.07

21.98 ± 6.96

0.57

10.0

30.27 ± 1.50

1.75 ± 0.19

17.26

800.0

1.53 ± 0.07

4.91 ± 0.46

0.32

100.0

13.76 ± 3.90

26.66 ± 9.33

0.52

    

1000.0

1.56 ± 0.24

4.81 ± 1.55

0.32

        

2000.0

1.87 ± 0.15

5.49 ± 0.84

0.34

  1. kcat and KM values were analyzed using GraphPad Prism 7.0. Data are represented as mean ± standard deviation from six replicates and the coefficient of variation is within 5%.
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