Figure 6 | Scientific Reports

Figure 6

From: Identification of ADS024, a newly characterized strain of Bacillus velezensis with direct Clostridiodes difficile killing and toxin degradation bio-activities

Figure 6

ADS024 CFS, and lyophilate degrade C. difficile toxin A and toxin B. Lane 1 shows a Novex Hi Mark pre-stained protein ladder. Toxin A (left) and toxin B (right) were incubated with reconstituted lyophilized ADS024 (lanes 2–7), or CFS (lane 11) in the amounts shown, and toxin proteolytic cleavage was detected by western blot to toxin A and toxin B, respectively. Reconstituted lyophilate was tested once using duplicate samples. Lane 12 and lane 13 use a CFS of Lot 2 and Lot 3, respectively. Images were captured with a Licor Odyssey gel imager. See full uncropped image of Lanes 1–13 in Supplementary Fig. 1 in the supplemental information.

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