Figure 8 | Scientific Reports

Figure 8

From: Identification of ADS024, a newly characterized strain of Bacillus velezensis with direct Clostridiodes difficile killing and toxin degradation bio-activities

Figure 8

ADS024 CFS and IPA extracts can inhibit translation and disrupt B. firmus cell membranes. (a) B. firmus was incubated with ADS024 CFS for 30 min and 24 h, and bacterial cytological profiling was used to visualize morphological changes. B. firmus cell membranes are shown in red, and B. firmus DNA is shown in blue. SYTOX Green fluorescence indicates permeabilization of the B. firmus cell membrane. (b) Similar experiment as in (a) except that ADS024 IPA extracts were used. (c) Similar experiment as in (a) except that E. coli was used in place of B. firmus. (d) Similar experiment as in (c) in which E. coli was exposed to ADS024 IPA extracts. White scale bar for all images is 1 micron. Experiments were conducted in duplicate.

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