Figure 1
Validation of vessels found in phase-contrast microtomography by comparing with histology. (A) Orthogonal views of a blood vessel in a 0.94 µm voxel size microtomography image. The orange cross-hair indicates the location of the selected orthogonal planes. The vessel is characterized both by hyperintense parts with collapsed blood vessel (magenta arrow) and hypointense parts with voids (yellow arrow). (B) Microscope image of an approximately matching region of the same sample. The section is 10 µm thick and was stained with the Klüver-Barrera technique. (C) Binary vessel mask (red) obtained with the proposed segmentation method is overlayed on the image shown in A. Note that capillaries are well segmented (yellow arrowheads). (D) Zoomed inset of A. (E) Zoomed inset of B. (F) Maximum intensity projection of the inset region shown in D across 28 µm depth, showing an extended trajectory of the capillaries. Scale bars = 100 µm.
