Figure 2


Restriction digestion of both cry gene. (A) NcoI and AhdI restriction of pCAMBIA1301 vector containing cry genes cassette; M: 10 kb molecular weight marker, Lanes 1: shows blank, Lane 2: shows pCAMBIA1301, Lane 3: shows ligation without ligase, Lane 4, 5: shows ligation of cry genes with pCAMBIA1301. (B) PCR amplification of both cry genes. M: 10 kb molecular weight marker, Lanes 1, 2: show cry3Bb1 gene (459 bp), Lane 3, 5: show cry3 gene (562 bp), Lane 4: shows a blank.