Table 1 Honeybees analyzed in this study.
Young | Old | Total | ||||||||
|---|---|---|---|---|---|---|---|---|---|---|
4 | 5 | 6 | 7 | 8 | 17 | 19 | 20 | 21 | ||
Spring | ||||||||||
Total n. of bees | – | – | 90 | 45 | 45 | 70 | 40 | 35 | 30 | 335 |
PCR analysis (P–N) | 6–84 | 8–37 | 4–41 | 46–24 | 34–6 | 27–8 | 25–5 | 150–205 | ||
Selected for NGS analysis (P–N) | 5–5 | 7–7 | 3–3 | 3†–7† | 3†–3† | 10†–8† | 7†–5† | 38–38 | ||
Autumn | ||||||||||
Total n. of bees | 45 | 90 | 90 | 90 | – | – | 90 | 90 | 43 | 538 |
PCR analysis (P–N) | 1–44 | 4–86 | 8–82 | 5–85 | 44–46 | 49–41 | 30–13 | 140–398 | ||
Selected for NGS analysis (P–N) | 1–1 | 4–4 | 8–8 | 2–2 | 10–10 | 10–10 | 10–10 | 45–45 | ||
