Figure 3

CT images at cellular-level resolution from laboratory-based X-ray microscopy with paraffin-mediated contrast enhancement. (a) Orthogonal CT slices from three different perspectives. These images are derived from the sample shown in Fig. 2a and in Table 1 (normal mouse). Conditions for the data collection are described in Methods. A renal corpuscle is indicated by an arrowhead. (b) Measurement of SBE. Magnified part of (a) around the renal corpuscle is represented with an opacity profile along the yellow line (about 7 μm). A further magnified part of CT slice around the yellow line is shown as an inset of the line profile. In the profile, red circles represent observed values of opacity. Obtained SBE from the curve fitting (blue line) was 0.56 μm. (c) Parts of renal corpuscle. Presented is the single renal corpuscle marked in (a). Magnified CT slices are shown from left to right around distal tubule (DT; section 826th/1222), afferent arteriole (AA; section 854th/1222) with macula densa (MD), efferent arteriole (EA; section 905th/1222), and proximal tubule (PT; section 969th/1222). Relevant parts are encircled by yellow broken lines. Putative nuclei are visible in all parts as white dots with sizes in the range of 3–5 μm; some of them were indicated by arrowheads. Scale bars: 5 μm.