Figure 6

Lipid classes do not discriminate the lipidome of NAS-IMI and H water buffalo milk. (A) Scree plot of the principal component analysis (PCA) representing the total variance (green line) and the single variance (blue line) explained for each component (PC index). (B) 3D scatter plot of the PCA representing sample distribution based on PC1, PC2, and PC3 of lipidomic profiles of healthy (pink) and NAS-IMI (cyan) milk samples. (C) Volcano plot representing down-, upregulated (red dot) and unchanged lipid classes (grey dots) in NAS-IMI compared to H buffalos. Significant lipid classes were considered with |log2 fold-change|> 1 and − log10 adjusted p value > 1.3.