Figure 4 | Scientific Reports

Figure 4

From: A high-content neuron imaging assay demonstrates inhibition of prion disease-associated neurotoxicity by an anti-prion protein antibody

Figure 4

ICSM18 is not inherently neurotoxic. Hippocampal neurons were treated with ICSM18 antibody and its isotype control, BRIC222, with and a positive control [0.05% w/v RML-infected brain homogenate (BH)] and negative (media only) control for 72 h. (a) NeuN (orange) stain and (b) and MAP2 (grey) overlaid with the neurite trace (white). (c) Magnified view of the white box in (b) showing a representative neuron nucleus and neurite root count. Images were run through analysis scripts that counted (d) neurons and (e) neurite fragmentation and calculated (f) neurite length, (g) root density and (h) branch level. Data are the percentage difference from the negative control and shown as mean ± SEM of n = 5 independent tests; one-way ANOVA with Dunnett’s multiple comparisons; ****P < 0.0001; ns not significant.

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