Figure 5 | Scientific Reports

Figure 5

From: A high-content neuron imaging assay demonstrates inhibition of prion disease-associated neurotoxicity by an anti-prion protein antibody

Figure 5

ICSM18 is not inherently synaptotoxic. Hippocampal neurons were treated with ICSM18 antibody and its isotype control, BRIC222, with and a positive control [0.05% w/v RML-infected brain homogenate (BH)] and negative (media only) control for 72 h. (a) Representative high-throughput images of cells stained with MAP2, spinophilin and NeuN to visualise dendrites, dendritic spines and neuronal nuclei respectively. (b) Dendritic spine image analysis mask (blue). (c,d) Magnified views of the white rectangles in (a) and (b). Images were run through analysis scripts that counted (e) dendritic spines and calculated (f) spine density; results are the percentage difference from the negative control and shown as mean ± SEM of n = 5 independent tests; one-way ANOVA with Dunnett’s multiple comparisons; ****P < 0.0001; ns not significant.

Back to article page