Figure 6 | Scientific Reports

Figure 6

From: A high-content neuron imaging assay demonstrates inhibition of prion disease-associated neurotoxicity by an anti-prion protein antibody

Figure 6

ICSM18 inhibits prion disease-associated neurotoxicity. Hippocampal neurons were treated with ICSM18 antibody and its isotype control, BRIC222 for 1.5 h before control uninfected or RML-infected brain homogenate (BH) was added to a final concentration of 0.01% w/v. Brain homogenate and antibodies were then co-incubated for a further 72 h before fixation and staining. (a) NeuN (orange) stain and (b) MAP2 (grey) overlaid with the neurite trace (white). (c) Magnified view of the white box in (b), showing a representative neuron nucleus and neurite root count. Images were run through analysis algorithms that counted (d) neurons and calculated (e) neurite length (f) branch level and (g) neuron root density. The baseline was removed on a per plate basis and then normalised between 0 and 100% on a batch basis in order to run non-linear regression and shown as mean ± SEM of n = 5 independent tests; non-linear regression analysis used the equation Y = 100/\({1} + {1}0^{{(({\text{LogEC}}_{{{5}0}} \, - \,{\text{X}})\, \times \,{\text{HillSlope}})}}\) and assumed antibody-negative RML treatment group as 0.05 nM.

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