Table 1 NCE1/2 values of p-TEMPO–Bn–Sc–peptides at their singly and doubly protonated states in MS/MS and MS3.

From: New free radical-initiated peptide sequencing (FRIPS) mass spectrometry reagent with high conjugation efficiency enabling single-step peptide sequencing

Peptide

NCE1/2

Singly protonated

Doubly protonated

MS/MS

MS3

Δ

MS/MS

MS3

Δ

Angiotensin II

23.9

22.9

1.0

13.7

13.4

0.3

Des-Pro2-bradykinin

23.0

21.6

1.4

13.0

12.0

1.0

Glycoprotein IIb fragment

24.7

23.8

0.9

15.3

14.5

0.8

Kinetensin

24.6

23.6

1.0

15.3

14.5

0.8

  1. *All NCE1/2 values are displayed in arbitrary unit.
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