Table 1 50% antinociceptive doses and interaction index of curcumin, metformin, and curcumin–metformin combination in phase II of mouse formalin test.

From: Curcumin and metformin synergistically modulate peripheral and central immune mechanisms of pain

Monotherapy

 

ED50 ± SEM (mg/kg)

  

Curcumin

 

82.8 ± 17.6

  

Metformin

 

248.9 ± 106.5

Combination therapy (1:1)

Ratio

ED50 add ± SEM (mg/kg)

ED50 mix ± SEM (mg/kg)

CI

CUR + MET

1:3

165.8 ± 62.0

39.6 ± 7.1***

0.24

Curcumin

 

41.4 ± 8.8

9.9 ± 1.8

 

Metformin

 

124.4 ± 53.2

29.7 ± 5.3

 
  1. The ED50 values were determined from linear regression analysis of the log dose–response curves. The theoretical ED50 (ED50 add) was calculated based on the dose–response curves of the curcumin and metformin monotherapy. The experimental ED50 (ED50 mix) was determined by the experimentally derived dose–response curve of the curcumin–metformin combination treatment. The ED50s are presented with their respective S.E.M. values.
  2. CI combination index.
  3. ***p < 0.001 compared to the ED50 add (student’s t-test).
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