Figure 4 | Scientific Reports

Figure 4

From: Maximum depth sequencing reveals an ON/OFF replication slippage switch and apparent in vivo selection for bifidobacterial pilus expression

Figure 4

G’s end phenomenon. The position of available restriction sites present in the double stranded chromosomal locus of bbr_0113 (A) and bbr_1889 (B) used to generate a newly 3’end, downstream the sequence of interest, of one of the two chromosomal DNA strands are indicated. Because this 3’ end incorporates a 14nt random tag and the complementary sequence of the NGS primer R1 during the first step of MDS, the corresponding NGS reads generated using R1 will sequence first the 14nt family tag, followed by the tract, and then the 8nt tag. When MDS targets a chromosomal strand containing a G-tract (using either AatII or ZraI), the NGS read generated with primer R1 causes a G’s end phenomenon, which does not occur for primer R2 (C). Conversely, when MDS focus on a chromosomal DNA strand containing a C-tract (MluI), the G’s end phenomenon occurs with R2 but not with R1 (D).

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