Figure 6 | Scientific Reports

Figure 6

From: Maximum depth sequencing reveals an ON/OFF replication slippage switch and apparent in vivo selection for bifidobacterial pilus expression

Figure 6

Replication slippage analysis by Maximum Depth Sequencing (MDS). The stacked frequency of inDel at G/C tract of bbr_0113 locus (A) and bbr_1889 locus (B) is indicated for each specific number of base(s) addition or omission, with the frequency of the annotated nt length being omitted. The MDS analysis reveals the distribution of InDel derived from only one of the chromosomal DNA strands specifying the G-tract (DNA coding strand) or the C-tract (non-coding DNA strand). The identity of the chromosomal DNA strand with the restriction site used for the MDS is indicated at the top. The general cultivation condition of UCC2003 for each sample (numbering above each stacked frequency bar) is shown at the bottom, with the specific cultivation conditions also indicated in SI Table S1. The condition ‘control’ refers to a control experiment involving chemically synthesized oligonucleotides specifying the region of interest encompassing either the forward or reverse chromosomal DNA strand of bbr_0113 or bbr_1889 locus.

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