Table 1 Quantitative PCR primer sequences.
Target group | Primer probe | Sequence (5′–3′) | Product (bp) |
|---|---|---|---|
Total fungi | ITS1F | CTTGGTCATTTAGAGGAAGTAA | 479 |
ITS2R | GCTGCGTTCTTCATCGATGC | ||
R. cerealis | RctubF4 | CCTAAAGAGTCTGGAGTAAGTC | 203 |
RctubR4 | GCTAGTGCGGTCAATGTATAG | ||
F. pseudograminearum | Fp_TEF1α.2F | AAAAATTACGACAAAGCCGTAAAAA | 82 |
Fp_TEF1α.2R | ACTCGACACGCGCCTGTTACCC | ||
Fp_TEF1α.2P | ACTCGACACGCGCCTGTTACCC |
