Figure 2 | Scientific Reports

Figure 2

From: CRISPR/Cas9 editing of the MYO7A gene in rhesus macaque embryos to generate a primate model of Usher syndrome type 1B

Figure 2

MYO7A genotype of TE biopsies from two embryos transferred into a recipient animal and resulted in a singleton pregnancy. (a) TE cell genotyping results of two expanded blastocysts selected for transfer to surrogate females. Double slashes denote omitted DNA sequence. Genotyping by NGS revealed E190 possessed a single G insertion in exon 3 of the MYO7A gene in 92.1% of sequencing reads, whereas 98.5% of the sequencing reads from E773 possessed an insertion and deletion in the target region. The single G insertion observed in E190 generates a premature stop codon (*). The mutation observed in E773 included a 50 bp deletion and a 17 bp insertion, with the net result being a change of 10 amino acids. (b) Ultrasound confirmed a singleton pregnancy at 28 days after transfer of E190 and E773. Yellow circle indicated implanted embryo.

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