Figure 3
From: Disease relevance of rare VPS13B missense variants for neurodevelopmental Cohen syndrome
Rescue experiment of Golgi fragmentation by co-overexpression of VPS13B missense variants. HeLa cells were transfected with a VPS13B-specific RNAi (siRNA #25721, ambion). 48 h post RNAi-transfection HeLa cells were transfected with the respective pcDNA3.1_VPS13B-mut constructs. 24 h later, cells were processed for immunostaining of VPS13B (green) and GM130 (red). Imaging occurred with confocal microscopy. Nuclei were stained using DAPI, scale bars 10 µm.
