Figure 3

HPLC chromatogram for pyriproxyfen and pyrethroids extracted from Olyset® Duo and Royal Guard® LLINs with reference to internal standard ‘dicyclohexyl phthalate (DCP). (A) Olyset® Duo active ingredients, pyriproxyfen and trans-permethrin and cis permethrin, measured by HPLC-diode array detector (DAD) at three-wavelength 226 (black), 232 (blue) and 254 (purple) nm in LLIN extraction solution. (B) Royal Guard® active ingredients; pyriproxyfen, and alpha-cypermethrin, were detected at the same three-wavelength in the sample solution following LLIN extraction.