Figure 1 | Scientific Reports

Figure 1

From: Establishment of well-differentiated camelid airway cultures to study Middle East respiratory syndrome coronavirus

Figure 1

Establishment and characterization of Bactrian camel and llama AEC cultures. (A) Immunofluorescence analysis showing the development of tight-junctions (ZO-1, white) and ciliogenesis (β-tubulin, red) in Bactrian camel and llama AEC cultures over time from 1-day to 4 weeks post ALI exposure. The cells were counterstained with DAPI (blue) to visualize the nuclei. (B,C) Ciliogenesis quantification of camel and llama AEC cultures overtime, respectively. Ciliation was quantified by measuring the area above a fluorescence intensity threshold of five random images acquired per condition. (D) Transepithelial electrical resistance (TEER) measurement of camel and llama AEC cultures overtime during the differentiation. (E) Epithelial morphology of ex vivo tissues (upper panel) and well-differentiated camel and llama AEC cultures (lower panel). (F) DPP4 expression in well-differentiated camel and llama AEC cultures, with Vero cells as a positive control. Scale bar is 20 µm.

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