Figure 4

Analysis of the engrafted H23BW-Ery-Lin-shBCL11A LV transduced HSPCs in NBSGW mice. (A) Schematic representation of the mouse transplantation experiment. CsH: cyclosporin H. (B) The percentage engraftment measured in the total BM cells isolated 15 weeks after transplantation. (C) Multilineage analysis in the human CD45 positive (hCD45⁺) cells after 15 weeks of transplantation. (D) The percentage of CD71⁺ ZsGreen⁺cells in the erythroid cells obtained from BM by ex vivo erythropoiesis. (E) Representative immunoblots (left) and the densitometric quantitation of BCL11A knockdown (right) in the flow-sorted ZsGreen⁺ erythroid cells (normalized to actin levels). (F) Intracellular HbF analysis by flow cytometry to determine the percentages of HbF⁺ cells in the terminally differentiated ZsGreen⁺ erythroid cells (left) and the graphical representation of the data (right). (G) HPLC analysis of the percentages of Gγ+Aγ chains in the terminally differentiated ZsGreen⁺ erythroid cells after differentiation (left) and the graphical representation of the data (right). All data are compared to shScr. All data are mean ± SD (n = 3). ns, not significant, *p < 0.05 and ** p < 0.01.