Figure 1 | Scientific Reports

Figure 1

From: Gut mucosa dissociation protocols influence cell type proportions and single-cell gene expression levels

Figure 1The alternative text for this image may have been generated using AI.

Study overview and datasets. (A) Schematic representation of the study set-up. Biopsies were sampled, either used fresh or cryopreserved, and dissociated using the one-step collagenase protocol with collagenase at 37 °C; the two-step collagenase with EDTA in step 1 and collagenase at 37 °C in step 2; or the three-step protease protocol, starting with 4 °C EDTA incubation in step 1, protease digestion in step 2, and a short collagenase digestion at room temperature in step 3. (B) Two datasets were generated to investigate: 1. the effect of cryopreservation and 2. the effect of different dissociation protocols on cell-type viability, composition, transcriptome, and functionality. IEL, intraepithelial cell; EC, epithelial cell. Colonoscopy pictogram was drawn by Gan Khoon Lay, https://thenounproject.com/term/colonoscopy/1250282/.

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