Figure 1

Summary of differentially expressed genes and the top Gene Ontology (GO) terms associated with significantly differentially expressed genes. Meloidogyne incognita second-stage juveniles were exposed to fluazaindolizine, fluensulfone, fluopyram, and oxamyl for 24-h and high throughput sequencing was used to determine gene expression compared to water control (N = 4 replicates/treatment). Volcano plots showing the differentially expressed genes in each treatment of this study (A–D). Dashed lines represent Log₂ Fold Change values of − 2 and 2 and the y-axis the − Log₁₀ of the adjusted p-value. The Venn diagram (E) shows the number of overlapping significantly (p-adjusted value < 0.05) differentially expressed genes between treatments. The number of up- or down-regulated genes, respectively (U and D). The top 15 Gene Ontology (GO) were examined in each treatment (F–I). The number of significantly (p-adjusted value < 0.05) differentially expressed genes in a treatment per a GO term are represented in the bar graphs. Color in each graph indicates the broader GO category each GO term belongs to.