Figure 2

Gene expression of transcription factors and enzymes involved with nematode xenobiotic detoxification. Meloidogyne incognita second-stage juveniles were exposed to fluazaindolizine, fluensulfone, fluopyram, and oxamyl for 24-h and high throughput sequencing used to determine gene expression compared to water control (N = 4 replicates/treatment). In each panel expression in the form of Log₂ Fold Change (Log₂FC) red indicates up-regulated expression compared to control, and blue indicates down-regulation. Expression of transcription factors in the Miskn1-like family (A) and Midaf16-like family (B) are shown with colored stars representing genes of interest (blue star—Miskn1-like-1 and green star—Midaf16-like-1). Average expression of differentially expressed genes at each step of nematode xenobiotic detoxification (C). The number significant up-regulated and down-regulated genes at each step are shown in the cells of panel (C), with down-regulated genes in the brackets. Asterisks throughout the figure represent significantly differentially expressed genes (p-adjusted value < 0.05).