Figure 2 | Scientific Reports

Figure 2

From: Genistein induces long-term expression of progesterone receptor regardless of estrogen receptor status and improves the prognosis of endometrial cancer patients

Figure 2Figure 2

Effects of genistein on the proliferation of cancer cells. The water-soluble tetrazolium salt-8 (WST-8) cell proliferation assays were performed for Ishikawa and KLE cells (A). The viability of cancer cells was determined every 24–48 h post-treatment with 20 μM genistein. Data are represented as the mean value (n = 12). Statistical significance was determined using a two-way analysis of variance (ANOVA). Cell cycle analysis was performed using flow cytometry. Cancer cells were collected, and flow cytometry analysis was performed 144 h after exposure to genistein. Cell distribution at each phase of the cell cycle (B). Data represent the mean ± standard error of the mean (n = 3). Sub-G1 population of both cells (C). Data represent the mean ± standard error of the mean (n = 3). Western blot analysis of proteins involved in the G2/M phase (phospho-histone H3 (Ser10), cdc2, phospho-cdc2 (Tyr15), cdc25, and phosho-cdc25 (Ser216)) and apoptosis (caspase 3 and cleaved caspase 3) after 48 h of genistein treatment (D). Full-length western blots are presented in Supplementary Fig. 3. P values are based on Student’s t-test. *P < 0.05; **P < 0.01.

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