Figure 3 | Scientific Reports

Figure 3

From: Genistein induces long-term expression of progesterone receptor regardless of estrogen receptor status and improves the prognosis of endometrial cancer patients

Figure 3Figure 3Figure 3Figure 3Figure 3

Effects of genistein on expression of estrogen receptor (ER)α, ERβ, progesterone receptor (PR)-AB, and PR-B. The expressions of ERα, ERβ, PR-AB, and PR-B were examined by quantitative polymerase chain reaction (qPCR) analysis in Ishikawa and KLE cells after exposure to 20 μM genistein, daidzein, glycitein, and equol (A). The expression of the hormone receptors in Ishikawa (B) and KLE cells (C) was measured using qPCR after 0, 3, 6, 24, 48, and 72 h of 20 μM genistein treatment without medium replacement. The receptor expressions were measured every 2 or 3 d for 6 d in Ishikawa cells with medium replacement every day (D) and measured every 1 or 2 d for 9 d in KLE cells (E). The relative expression values are displayed in logarithms with a base of 2. Data represent the mean ± standard error of the mean (n = 3). Western blot analysis of these hormone receptors, forkhead box protein O1 (FOXO1), and insulin-like growth factor-binding protein 1 (IGFBP1) in cancer cells treated with genistein for 144 h (F). Full-length western blots are presented in Supplementary Fig. 4. Data represent the mean ± standard error of the mean (n = 3). P values are based on Student’s t-test. *P < 0.05; **P < 0.01.

Back to article page