Figure 8
(a) Representative microscopic images of Hoechst33342- and AO-stained HCT-116 cells. Scale bar = 50 µm. Chloroquine was used as a reference. (b) Quantification of microscopic images, presenting the integrated red fluorescence intensity, depicted as mean ± SEM of data obtained from 10 random fields of view. (c,d,f) The expression of various proteins detected by Western blot assay after HCT-116 treatment with 20d or DMSO. The level of each protein was normalized to that of β-actin. Error bars represent the data ± SEM (e) Representative microscopic images of HCT-116 cells. LC3B is depicted in green and nucleus in blue (DAPI). Scale bar = 10 µm (g) Quantification of microscopic images showing the integrated density of LC3B presented as mean ± SEM of data obtained from 10 random fields of view.
