Figure 1

Workflow used to study proteomic analysis of AT in HEF versus LEF dairy cows. AT was isolated from HEF and LEF groups, extracted proteins from samples. Proteins were digested and peptides were identified for the differential expression. Bioinformatic analysis and network analysis was performed to identify the FE related pathways generated by IPA (Qiagen). MS/MS spectra analysis confirmed the presence of peptides in the sequences of FE related proteins.