Figure 1
Cell viability with respect to concentration and time after DA exposure. (A) Cell viability decreased significantly at Day 1 in airway epithelial cultures exposed to DA concentrations of 8.6 mM and 11.4 mM, but not 5.7 mM DA, compared to PBS-exposed control cultures. MTT was measured within each culture well (n = 4/group) and calculated as a percentage change relative to non-exposed control wells. (B) Cell viability with respect to time after exposure (Days 1, 2, and 4) in non-exposed controls (black columns), 5.7 mM DA cultures (red columns), and 8.6 mM DA cultures (blue columns). Cell viability did not differ significantly in 5.7 mM DA-exposed cultures from PBS controls at any time point assessed. In 8.6 mM DA exposures, cell viability differed significantly at Days 1, 2 and 4 compared to controls (ANOVA with Dunnett’s correction, *p < 0.05, ***p < 0.001 and ****p < 0.0001). (C) Representative brightfield microscopy images of 16HBE cells at Day 2 and 4 after DA exposure. At Day 4, cell density improved following 5.7 mM DA exposure whereas 8.6 mM-exposed cells showed reduced cell density.
