Figure 3
From: Microliter-scale reaction arrays for economical high-throughput experimentation in radiochemistry
Experimental set up for one batch of experiments that explored the influence of reaction temperature (8 values) and solvent (2 types) for the synthesis of [18F]Flumazenil. (A) Assignment of 64 reaction sites. Half of the reaction sites were used first to explore 4 different temperatures in the first set of 32 simultaneous reactions. Then the other half of the sites were used for the remaining 4 temperatures. (B) Cerenkov images show the distribution of the residual activity on each chip after collecting the crude products. Radioactivity signal is decay-corrected to a common timepoint for all images. The reaction marked with an “X” was not analyzed (by mistake the precursor droplet was not added to reaction site). (C) Cerenkov images of developed TLC plates (each containing 8 samples) for reactions that used DMSO as the reaction solvent. (D) Separated crude samples using DMF as the reaction solvent. Dashed circles indicate the ROIs used for analysis. The dashed red arrow indicates the direction of solvent movement during development. White dotted lines represent the boundary of each multi-sample plate.
