Figure 2 | Scientific Reports

Figure 2

From: Global O-GlcNAcylation changes impact desmin phosphorylation and its partition toward cytoskeleton in C2C12 skeletal muscle cells differentiated into myotubes

Figure 2

Quantification of global O-GlcNAcylation level in C2C12 myotubes following Ac4-5S-GlcNAc (5S, ▲) or Thiamet G (ThG, ■) treatment. Twenty µg of proteins sample extracted with RIPA buffer were resolved on 7.5% acrylamide Stain-free gels and analysed by western blot. (a) Whole protein profiles detected using Stain-Free technology. (b) O-GlcNAcylation pattern revealed with RL-2 antibody. (c) Quantification of western blot signals, normalized to Stain-Free profile. Data were expressed as mean ± SEM and compared with the control condition. *p < 0.05, **p < 0.01 significantly different from control (n = 15, N = 3 independent cultures). Uncropped images of gel and blot are presented in Supplemental data.

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