Figure 6
Quantification without an a priori of phosphatase activities involved in the desmin dephosphorylation. (a) In-gel detection of phosphatases activity after separation of 75 µg of proteins extracted from untreated myotubes (C, ●), and Ac4-5S-GlcNAc- and Thiamet G-treated myotubes (5S, ▲ and ThG, ■ respectively) using 10% Native-PAGE; MUP was used as phosphatase substrate, leading to fluorescence emission once hydrolysed by phosphatases. The Calf Intestine Phosphatase (CIP, 1 mU) was used as positive control. (b) Quantification of total phosphatases activities. Resulting signals were quantified, expressed as mean ± SEM and compared with the control condition (n = 15; N = 3 independent cultures). Uncropped image of gel is presented in Supplemental data.
