Figure 8 | Scientific Reports

Figure 8

From: Global O-GlcNAcylation changes impact desmin phosphorylation and its partition toward cytoskeleton in C2C12 skeletal muscle cells differentiated into myotubes

Figure 8

Quantification of the phosphorylated forms of desmin in C2C12 differentiated myotubes consecutively to co-treatment caffeine and KN-93, CamKII activator and inhibitor, respectively. Twenty µg of the whole proteins samples were separated using Phos-tag-PAGE, and desmin was detected using western blot. (a) Visualization of phosphorylated pattern of desmin in untreated (C), caffeine (Caf) and caffeine + KN-93 (Caf + KN-93) treated myotubes. The phosphorylated forms of desmin were separated according to the number of phosphate groups (P1: first retarded form of desmin; P2: second retarded form of desmin) comparing with the non-phosphorylated form of desmin (NP). (b) The signals were quantified and expressed as the relative percentage of each form (NP, P1 and P2 forms, comparing with the sum of all forms (NP + P1 + P2, equal to 100%, for each condition). ***p < 0.001: significantly different from control (n = 15; N = 3 independent cultures). Uncropped image of blot is presented in Supplemental data.

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